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Image Search Results
Journal: Science Advances
Article Title: Zeolitic imidazolate frameworks activate endosomal Toll-like receptors and potentiate immunogenicity of SARS-CoV-2 spike protein trimer
doi: 10.1126/sciadv.adj6380
Figure Lengend Snippet: ( A ) C57BL/6 mice were intradermally injected with either soluble dye (XenoLight DiR) or D-ZIF. ICP-MS analysis of Zn 2+ in dLN isolated 3 and 24 hours after intradermal injection with soluble dye or D-ZIF showing the increase in Zn 2+ in dLN in D-ZIF–treated mice. ppb, parts per billion. ( B to E ) Flow cytometry quantification of CD45 + CD11c + (B), pDCs (C), cDC + MHC II + (D), and cDC + CD8 + (E) cells in dLNs 1, 3, and 24 hours after injection showing the time-dependent increase in the influx of different subsets of DCs. Data are shown as means ± SEM. n = 3 or 4. Statistical significance was calculated by unpaired two-tailed Student’s t test or one-way analysis of variance (ANOVA): * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.
Article Snippet: Cells were centrifuged and resuspended in a buffer containing zombie violet, CD45, CD11c (Tonbo Biosciences, 910004), CD11b (Tonbo Biosciences, 910004),
Techniques: Injection, Isolation, Flow Cytometry, Two Tailed Test